陈伟业，男，博士，副研究员，硕士生导师，重要人兽共患病与烈性外来病创新团队，研究领域为小反刍兽疫的综合防控，包括小反刍兽疫重组山羊痘病毒活载体疫苗、小反刍兽致病机制等方面的研究。作为主持人和主要执行人参与了国家国际合作专项、国家自然科学基金、国家高技术研究发展计划、国家重点研发计划等10余项科研项目。发表SCI论文30余篇，其中第一作者或通讯作者6篇。培养硕士研究生4人。

 

（1）研究方向：动物疫苗与分子免疫学

（2）电话：0451-51051725

（3）邮箱：chenweiye@caas.cn

（4）通讯地址：黑龙江省哈尔滨市香坊区哈平路678号，哈尔滨兽医研究所，150069

 

1998.09-2002.06  南京农业大学   兽医学         学士 

2002.09-2007.06  南京农业大学   临床兽医学    “硕博连读”获得博士学位

 

2007.07-2010.09    中国农科院哈尔滨兽医研究所  博士后/副研究员

2010.09-至今      中国农科院哈尔滨兽医研究所   副研究员

 

   1. 国家国际合作专项：小反刍兽疫新型疫苗创制（2015DFA31300，2015.04-2018.03, 248万元；

   2. 国家重点研发计划：小反刍兽疫病毒感染与免疫调控机制研究（2016YFD0500108，2016.05-2020.12）, 140万元；

   3. 国家自然科学基金青年基金：小反刍兽疫病毒反向遗传操作平台建立及标记疫苗构建（30901082，2010.01-2012.12）, 20万元；

   4. 国家高技术研究发展计划：山羊痘病毒载体小反刍兽疫疫苗的研制和评价（2011AA10A208，2011.01-2015.12）, 125万元；

   5. 国家科技重大专项：动物布尼亚病原谱流行规律研究（2012ZX10004214-002-007，2012.01 -2015.12）, 22.67万元；

   6. 公益性行业（农业）科研专项：新型动物疫病诊断技术的应用研究（201203056，2012.01 -2016.12）, 10万元；

 

1.Chen, W., Wen, Z., Zhang, J., Li, C., Huang, K. and Bu, Z., 2017. Establishing a safe, rapid, convenient and low-cost antiviral assay of interferon bioactivity based on recombinant VSV expressing GFP. J Virol Methods 252, 1-7. 

2.Zhang, J., W. Liu, W. Chen*, C. Li, M. Xie, and Z. Bu. 2016. Development of an Immunoperoxidase Monolayer Assay for the Detection of Antibodies against Peste des Petits Ruminants Virus Based on BHK-21 Cell Line Stably Expressing the Goat Signaling Lymphocyte Activation Molecule. PLoS One 11:e0165088. 

3.Yin, C., Chen, W., Hu, Q., Wen, Z., Wang, X., Ge, J., Yin, Q., Zhi, H., Xia, C., Bu, Z., 2014, Induction of protective immune response against both PPRV and FMDV by a novel recombinant PPRV expressing FMDV VP1. Veterinary Research 45, 62. 

4.Hu, Q., Chen, W.*, Huang, K., Baron, M.D., Bu, Z., 2012, Rescue of recombinant peste des petits ruminants virus: creation of a GFP-expressing virus and application in rapid virus neutralization test. Vet Res 43, 48. 

5.Wen, Z., Wu, C., Chen, W., Zeng, X., Shi, J., Ge, J., Chen, H., and Bu, Z. (2015). Establishment of MDCK Stable Cell Lines Expressing TMPRSS2 and MSPL and Their Applications in Propagating Influenza Vaccine Viruses in Absence of Exogenous Trypsin. Biotechnol Res Int 2015, 402628. 

6.Chen, W., Hu, S., Qu, L., Hu, Q., Zhang, Q., Zhi, H., Huang, K., Bu, Z., 2010, A goat poxvirus-vectored peste-des-petits-ruminants vaccine induces long-lasting neutralization antibody to high levels in goats and sheep. Vaccine 28(30), 4742-4750.

7.Chen, W., Cao, W., Zhao, H., Hu, Q., Qu, L., Hu, S., Ge, J., Wen, Z., Wang, X., Li, H., Huang, K., Bu, Z., 2011, Establishment of a stable CHO cell line with high level expression of recombinant porcine IFN-beta. Cytokine 54, 324-329. 

8.Kong, D., Wen, Z., Su, H., Ge, J., Chen, W., Wang, X., Wu, C., Yang, C., Chen, H., Bu, Z., 2012, Newcastle disease virus-vectored Nipah encephalitis vaccines induce B and T cell responses in mice and long-lasting neutralizing antibodies in pigs. Virology 432（2）, 327-335.

9.Liu, W.X., Hu, S., Qiao, Z.J., Chen, W.Y., Liu, L.T., Wang, F.K., Hua, R.H., Bu, Z.G., Li, X.R., 2011, Expression, purification, and improved antigenic specificity of a truncated recombinant bp26 protein of Brucella melitensis M5-90: a potential antigen for differential serodiagnosis of brucellosis in sheep and goats. Biotechnol Appl Biochem 58, 32-38.

10.Hua, R.H., Chen, N.S., Qin, C.F., Deng, Y.Q., Ge, J.Y., Wang, X.J., Qiao, Z.J., Chen, W.Y., Wen, Z.Y., Liu, W.X., Hu, S., Bu, Z.G., 2010, Identification and characterization of a virus-specific continuous B-cell epitope on the PrM/M protein of Japanese Encephalitis Virus: potential application in the detection of antibodies to distinguish Japanese Encephalitis Virus infection from West Nile Virus and Dengue Virus infections. Virol J 7, 249.

11.Bai, Y., Tong, T., Liu, G., Chen, W., Zhang, W., Wang, Q., Yang, T., Bu, Z., Wu, D., 2009, Expression of biologically active recombinant equine interferon-gamma in Escherichia coli. Comp Immunol Microbiol Infect Dis 33, 333-342.

12.Zhu, Q., Yang, H., Chen, W., Cao, W., Zhong, G., Jiao, P., Deng, G., Yu, K., Yang, C., Bu, Z., Kawaoka, Y., Chen, H., 2008, A naturally occurring deletion in its NS gene contributes to the attenuation of an H5N1 swine influenza virus in chickens. J Virol 82, 220-228.

 

1.步志高，陈伟业，基于IPMA的小反刍兽疫病毒抗体检测试剂盒, 201510324242.0

2.步志高，陈伟业，表达融合外源表位N蛋白的重组小反刍兽疫病毒的构建及应用, 201310182866.4

3.步志高，陈伟业，小反刍兽疫病毒反向遗传操作系统及应用, 201010559545.8

4.步志高，陈伟业，胡森，小反刍兽疫重组山羊痘病毒活载体疫苗, 201110265443.X

5.步志高，胡森，陈伟业，小反刍兽疫重组山羊痘病毒活载体疫苗，200810166154.2

 

1. 小反刍兽疫防控关键技术的创建及应用，神农中华农业科技奖科研类成果一等奖，支海兵，才学鹏，薛青红，印春生，步志高，冯忠泽，窦永喜，陈伟业，夏业才，蒙学莲，李宁，王乐元，孙晔，黄炯，李延涛，努尔.库尔玛那里，王玉红，张德坤，次真，田野，2016-2017年度