2、联系方式

（1）研究领域：动物细菌病

（2）研究方向：食源性致病菌与食品安全研究

（3）电话：0451-51051733

（4）邮箱：yushenye@caas.cn

（5）通讯地址：黑龙江省哈尔滨市香坊区哈平路678号哈尔滨兽医研究所综合楼5014，150069

 

3、教育经历 

1999.09-2003.06   山东农业大学     水产养殖学专业      学士

2003.09-2006.06   山东农业大学     预防兽医学专业      硕士

2006.09-2009.06   吉林大学         预防兽医学专业      博士

 

4、工作经历

2009.07-2009.12       保定市金诺兽药研究所   研究实习员

2010.01-2014.12       保定市金诺兽药研究所   助理研究员

2015.01 至今         保定市金诺兽药研究所   副研究员

 

5、承担项目

1)“十三五” 国家重点研发计划：种禽场沙门氏菌病综合防控技术集成与示范研究（2016YFD0501608，2016.07-2020.12），50万元；

2)国家自然科学基金面上项目：大肠杆菌phiX174噬菌体E蛋白诱导菌蜕形成的机制研究（31470893，2015.01-2018.12），85万元；

3)国家自然科学基金青年基金：时序性多靶位溶菌系统的构建及E基因突变体裂解机制初步解析（31100659，2012.01-2014.12），23万元；

4)兽医生物技术国家重点实验室自主研究课题：SRRz裂解盒与E基因突变体协同溶菌机制研究（SKLVBP201216，2012.01-2012.12），10万元

 

6、代表论文与着作

1)Qiufeng Tian, Wei Zhou, Wei Si, Fei Yi, Xin Hua, Min Yue, Liping Chen, Siguo Liu*, Shenye Yu*. Construction of Salmonella Pullorum ghost by co-expression of lysis gene E and the antimicrobial peptide SMAP29 and evaluation of its immune efficacy in specific-pathogen-free chicks. Journal of Integrative Agriculture. 2017, accepted.

2)Jun Cao, Fei Yi, Qiufeng Tian, Guanghui Dang, Wei Si, Siguo Liu*, Shenye Yu*. Targeting the gram-negative bacteria peptidoglycan synthase MraY as a new approach for monoclonal antibody anti-bacterial activity. Human Vaccines & Immunotherapeutics. 2017, 13(9):2086-2091.

3)Shenye Yu, Hailing Zhao, Huanan Wang, Xiumei Wang, Guangxi Shao, Lili Xu, Wei Si, Liping Chen, Wanjiang Zhang, Siguo Liu*. Production and characterization of mouse monoclonal antibodies against lysis protein E of phiX174. Journal of Virological Methods. 2013, 189(2):355-361.

4)Shenye Yu, Wei Peng, Wei Si, Lu Yin, Siguo Liu*, Huifang Liu, Hailing Zhao, Chunlai Wang, Yuehong Chang, Yuezhi Lin. Enhancement of bacteriolysis of shuffled phage PhiX174 gene E. Virology Journal. 2011, 8:206.

5)Wei Si, Shenye Yu, Henggui Liu, Chunlai Wang, Liping Chen, Gaoling Wang, Siguo Liu*. A bacterial ghost improves the immunological efficacy of a Newcastle disease virus inactivated vaccine. Veterinary Microbiology. 2017, 203:189-195.

6)Wei Si, Xiumei Wang, Huifang Liu, Shenye Yu, Zhaoli Li, Liping Chen, Wanjiang Zhang, Siguo Liu*. Physiology, pathogenicity and immunogenicity of live, attenuated Salmonella enterica serovar Enteritidis mutants in chicks. Microbial Pathogenesis. 2015, 83-84:6-11.

7)Bin Tang, Mingyuan Liu, Libo Wang, Shenye Yu, Haining Shi, Pascal Boireau, Vasile Cozma, Xiuping Wu*, Xiaolei Liu*. Characterisation of a high-frequency gene encoding a strongly antigenic cystatin-like protein from Trichinella spiralis at its early invasion stage. Parasites & Vectors. 2015, 8:78.

8)Shuang Lv, Wei Si, Shenye Yu, Zhaoli Li, Xiumei Wang, Liping Chen, Wanjiang Zhang, Siguo Liu*. Characteristics of invasion-reduced hilA gene mutant of Salmonella Enteritidis in vitro and in vivo. Research in Veterinary Science. 2015, 101:63-68.

9)Wei Si, Shenye Yu, Liping Chen, Xiumei Wang, Wanjiang Zhang, Siguo Liu*, Guangxing Li*. Passive protection against Salmonella enterica serovar Enteritidis infection from maternally derived antibodies of hens vaccinated with a ghost vaccine. Research in Veterinary Science. 2014, 97(2):191-193.

10)Wanjiang Zhang, Zongji Lu, Stefan Schwarz, Rongmin Zhang, Xiumei Wang, Wei Si, Shenye Yu, Liping Chen, Siguo Liu*. Complete sequence of the bla(NDM-1)-carrying plasmid pNDM-AB from Acinetobacter baumannii of food animal origin. The Journal of Antimicrobial Chemotherapy. 2013, 68(7):1681-1682.

11)Huanan Wang, Ting Zhu, Shenye Yu, Huifang Liu, Xiumei Wang, Liping Chen, Wei Si, Hai Pang*, Siguo Liu*. A novel B cell epitope in cold-shock DEAD-box protein A from Mycobacterium tuberculosis. Research in Veterinary Science. 2013, 94(3):406-412.

12)Xiumei Wang, Xingfeng Lü, Lu Yin, Huifang Liu, Wanjiang Zhang, Wei Si, Shenye Yu, Meili Shao, Siguo Liu*. Occurrence and antimicrobial susceptibility of Listeria monocytogenes isolates from retail raw foods. Food Control. 2013, 32(1):153-158.

13)Hailing Zhao, Shenye Yu, Huifang Liu, Wei Si, Chunlai Wang, Siguo Liu*. Identification of antigenic epitopes of the SapA protein of Campylobacter fetus using a phage display peptide library. Research in Veterinary Science. 2012, 93(3):1274-1280.

14)Xiumei Wang, Wanjiang Zhang, Stefan Schwarz, Shenye Yu, Huifang Liu, Wei Si, Rongmin Zhang, Siguo Liu*. Methicillin-resistant Staphylococcus aureus ST9 from a case of bovine mastitis carries the genes cfr and erm(A) on a small plasmid. The Journal of Antimicrobial Chemotherapy. 2012, 67(5):1287-1289.

15)Wei Peng, Wei Si, Lu Yin, Huifang Liu, Shenye Yu, Siguo Liu*, Chunlai Wang, Yuehong Chang, Zhuo Zhang, Shouping Hu, Yanfen Du. Salmonella enteritidis ghost vaccine induces effective protection against lethal challenge in specific-pathogen-free chicks. Immunobiology. 2011, 216(5):558-565.

 

7、专利和标准

1)于申业，刘思国，抗大肠杆菌感染的治疗性单克隆抗体、产生该单克隆抗体的杂交瘤细胞株及其用途，ZL201410412146.7

2)于申业，刘思国，抗phiX174噬菌体裂解蛋白E的单克隆抗体、产生该抗体的杂交瘤细胞株及其表位，ZL201210001050.2

3)刘思国，黎观红，贾永杰，洪智敏，司微，于申业，一种乳酸杆菌细胞壁完整肽聚糖及其在提高IEC抗菌肽表达水平中的应用，ZL201210122033.4

4)刘思国，王华南，于申业，朱婷，于秀婷，刘慧芳，王秀梅，司微，抗结核分枝杆菌单克隆抗体及其识别的抗原表位和用途，ZL201110269165.5

5)刘思国，刘慧芳，王秀梅，于申业，王牟平，司微，杨志，减毒沙门氏菌及其应用，ZL201110061628.9

 

8、获得奖励

1)牛结核病病原生态学和流行病学研究，中国农业科学院自然科学二等奖，刘思国，刘慧芳，司微，王春来，于申业，王牟平，2012年。

2)牛结核病病原生态学和流行病学研究，黑龙江省自然科学三等奖，刘思国，司微，于申业，王牟平，陈利苹，2014年。

3)副猪嗜血杆菌菌影的制备，黑龙江省自然科学技术学术成果二等奖，王春来，胡明明，常月红，张艳禾，司微，谢芳，于申业，刘慧芳，刘思国，沈楠，2013年。